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Table 1 Optimization parameters for decolorize the MG textile dye by R. mucilaginosa AUMC13567

From: Bioremediation of malachite green dye toxicity under optimized conditions by Rhodotorula mucilaginosa AUMC13567

a] Effect of three types of growth media

b] Type of Incubation

c] Agitation speed

Incubation by hours

a)Plain water

b) Glucose

c) Media

Static

Submerged

50rpm

80rpm

100rpm

120rpm

150rpm

F-value

2hrs

3.8 ± 0.1a1

12.3 ± 0.2 b1

15.3 ± 0.2 c1

7.7 ± 0.21

15.3 ± 0.2 1

4.8 ± 0.5a1

5.9 ± 0.1 b1

26.2 ± 0.1 c1

42.9 ± 0.4 d1

60.7 ± 0.0e1

7050***

4hrs

5.6 ± 0.6a1

14.1 ± 0.2 b1

26.0 ± 0.4 c2

11.7 ± 0.32

26.0 ± 0.4 2

7.8 ± 0.2a2

15.0 ± 5.9 a2

27.6 ± 0.2 b1

49.4 ± 0.7c2

80.4 ± 0.1d2

120***

6hrs

10.8 ± 0.3a2

21.8 ± 1.9 b2

55.9 ± 0.2c3

45.9 ± 0.13

55.9 ± 0.2 3

11.0 ± 0.8a3

11.2 ± 1.3 a3

61.0 ± 0.1 b2

54.2 ± 0.2c3

96.4 ± 0.1 d3

2669***

8hrs

20.4 ± 2.0a3

37.5 ± 0.6 b3

75.5 ± 1.0 c4

51.1 ± 1.14

75.5 ± 1.0 4

16.7 ± 0.2a4

16.7 ± 0.3 a4

68.9 ± 1.0 b3

57.0 ± 0.8c4

97.8 ± 0.0 d4

3717***

10hrs

41.8 ± 0.1a4

50.5 ± 0.11b4

87.0 ± 0.3 c5

54.1 ± 1.05

87.0 ± 0.3 5

25.4 ± 0.2a5

27.1 ± 0.3b5

87.4 ± 0.4 c4

67.2 ± 0.1d5

99.5 ± 0.0 e5

26661***

12hrs

70.7 ± 0.6a5

83.3 ± 0.1b5

92.0 ± 0.0c6

56.0 ± 0.26

92.0 ± 0.06

29.2 ± 0.1a6

32.0 ± 0.1 a6

94.1 ± 1.2 b5

95.8 ± 1.9b6

100 ± 0.0 c6

1310***

F-value

854***

4568***

1091***

1238

4568

437***

312***

2503***

442***

97832***

 

Completely decolorize

56hr

32hr

24hr

50 hrs.

24hr

76h

72h

30 h

24 h

  

d] Incubation temperature degree

e] Malachite Green Dye Concentrations

Incubation by hours

25Ëšc

28 Ëšc

30 Ëšc

37 Ëšc

F-value

50mg

100mg

200mg

300mg

500mg

1000mg

F-value

2hrs

7.0 ± 0.1a1

15.3 ± 0.2 b1

26.2 ± 0.1 c1

60.7 ± 0.0 d1

28006***

60.7 ± 0.0e1

54.2 ± 0.9 d1

4.5 ± 0.0 b1

9.6 ± 0.0 c1

0.2 ± 0.1a1

0.0 ± 0.0 a

5931***

4hrs

12.4 ± 0.1a2

26.0 ± 0.4 b2

27.6 ± 0.2 c1

80.4 ± 0.1 d2

15418***

80.4 ± 0.1d2

81.6 ± 0.1 e2

12.8 ± 0.0 c2

13.0 ± 0.1c2

1.9 ± 0.0b2

0.0 ± 0.0e

299943***

6hrs

52.5 ± 0.1a3

52.6 ± 0.4 a3

61.0 ± 0.1 b2

96.4 ± 0.1 c3

12466***

96.4 ± 0.1f3

92.2 ± 0.2 e3

15.8 ± 0.0d3

13.7 ± 0.0c3

3.5 ± 0.0 b3

0.0 ± 0.0a

156052***

8hrs

56.9 ± 0.1a4

62.3 ± 0.8 b4

68.9 ± 1.0 c3

97.8 ± 0.0 d4

833***

97.8 ± 0.0e4

97.9 ± 0.1e4

18.3 ± 0.1 d4

14.4 ± 0.0c4

3.5 ± 0.0 b3

0.0 ± 0.0 e

541877***

10hrs

59.1 ± 0.0a5

87.0 ± 0.3 b5

87.4 ± 0.4 b4

99.5 ± 0.0 c5

4225***

99.5 ± 0.0e5

99.6 ± 0.0e5

28.2 ± 0.7 d5

14.4 ± 0.1c4

3.5 ± 0.0 b3

0.0 ± 0.0 a

27423***

12hrs

66.5 ± 0.3a6

92.0 ± 0.0 b6

94.1 ± 1.2 c5

100 ± 0.0d6

575***

100 ± 0.0e6

99.7 ± 0.0e5

32.5 ± 1.0 d6

14.4 ± 0.1c4

3.5 ± 0.0b3

0.0 ± 0.0 a

12754***

F-value

35472***

5096***

1952***

97832***

 

97832**

2184***

418***

663***

2413***

  

Completely decolorize

72hr

30hr

24hr

   

After 13hrs

After 72hrs

    
  1. All results are compared to each other at P < 0.05. Values with the different superscripts along the same column are statistically different from each other ***p < 0.001. by One Way Anova values comparison. Post Hoc Tests: Duncan a p < 0.05 is represented by superscripts 1–10 and different superscripts along the same row are statistically different from each other ***p < 0.001. by One Way Anova values comparison. Post Hoc Tests: Duncan a p < 0.05 is represented by superscripts a-f