a] Effect of three types of growth media | b] Type of Incubation | c] Agitation speed | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Incubation by hours | a)Plain water | b) Glucose | c) Media | Static | Submerged | 50rpm | 80rpm | 100rpm | 120rpm | 150rpm | F-value | |
2hrs | 3.8 ± 0.1a1 | 12.3 ± 0.2 b1 | 15.3 ± 0.2 c1 | 7.7 ± 0.21 | 15.3 ± 0.2 1 | 4.8 ± 0.5a1 | 5.9 ± 0.1 b1 | 26.2 ± 0.1 c1 | 42.9 ± 0.4 d1 | 60.7 ± 0.0e1 | 7050*** | |
4hrs | 5.6 ± 0.6a1 | 14.1 ± 0.2 b1 | 26.0 ± 0.4 c2 | 11.7 ± 0.32 | 26.0 ± 0.4 2 | 7.8 ± 0.2a2 | 15.0 ± 5.9 a2 | 27.6 ± 0.2 b1 | 49.4 ± 0.7c2 | 80.4 ± 0.1d2 | 120*** | |
6hrs | 10.8 ± 0.3a2 | 21.8 ± 1.9 b2 | 55.9 ± 0.2c3 | 45.9 ± 0.13 | 55.9 ± 0.2 3 | 11.0 ± 0.8a3 | 11.2 ± 1.3 a3 | 61.0 ± 0.1 b2 | 54.2 ± 0.2c3 | 96.4 ± 0.1 d3 | 2669*** | |
8hrs | 20.4 ± 2.0a3 | 37.5 ± 0.6 b3 | 75.5 ± 1.0 c4 | 51.1 ± 1.14 | 75.5 ± 1.0 4 | 16.7 ± 0.2a4 | 16.7 ± 0.3 a4 | 68.9 ± 1.0 b3 | 57.0 ± 0.8c4 | 97.8 ± 0.0 d4 | 3717*** | |
10hrs | 41.8 ± 0.1a4 | 50.5 ± 0.11b4 | 87.0 ± 0.3 c5 | 54.1 ± 1.05 | 87.0 ± 0.3 5 | 25.4 ± 0.2a5 | 27.1 ± 0.3b5 | 87.4 ± 0.4 c4 | 67.2 ± 0.1d5 | 99.5 ± 0.0 e5 | 26661*** | |
12hrs | 70.7 ± 0.6a5 | 83.3 ± 0.1b5 | 92.0 ± 0.0c6 | 56.0 ± 0.26 | 92.0 ± 0.06 | 29.2 ± 0.1a6 | 32.0 ± 0.1 a6 | 94.1 ± 1.2 b5 | 95.8 ± 1.9b6 | 100 ± 0.0 c6 | 1310*** | |
F-value | 854*** | 4568*** | 1091*** | 1238 | 4568 | 437*** | 312*** | 2503*** | 442*** | 97832*** | Â | |
Completely decolorize | 56hr | 32hr | 24hr | 50 hrs. | 24hr | 76h | 72h | 30 h | 24 h | Â | Â | |
d] Incubation temperature degree | e] Malachite Green Dye Concentrations | |||||||||||
Incubation by hours | 25Ëšc | 28 Ëšc | 30 Ëšc | 37 Ëšc | F-value | 50mg | 100mg | 200mg | 300mg | 500mg | 1000mg | F-value |
2hrs | 7.0 ± 0.1a1 | 15.3 ± 0.2 b1 | 26.2 ± 0.1 c1 | 60.7 ± 0.0 d1 | 28006*** | 60.7 ± 0.0e1 | 54.2 ± 0.9 d1 | 4.5 ± 0.0 b1 | 9.6 ± 0.0 c1 | 0.2 ± 0.1a1 | 0.0 ± 0.0 a | 5931*** |
4hrs | 12.4 ± 0.1a2 | 26.0 ± 0.4 b2 | 27.6 ± 0.2 c1 | 80.4 ± 0.1 d2 | 15418*** | 80.4 ± 0.1d2 | 81.6 ± 0.1 e2 | 12.8 ± 0.0 c2 | 13.0 ± 0.1c2 | 1.9 ± 0.0b2 | 0.0 ± 0.0e | 299943*** |
6hrs | 52.5 ± 0.1a3 | 52.6 ± 0.4 a3 | 61.0 ± 0.1 b2 | 96.4 ± 0.1 c3 | 12466*** | 96.4 ± 0.1f3 | 92.2 ± 0.2 e3 | 15.8 ± 0.0d3 | 13.7 ± 0.0c3 | 3.5 ± 0.0 b3 | 0.0 ± 0.0a | 156052*** |
8hrs | 56.9 ± 0.1a4 | 62.3 ± 0.8 b4 | 68.9 ± 1.0 c3 | 97.8 ± 0.0 d4 | 833*** | 97.8 ± 0.0e4 | 97.9 ± 0.1e4 | 18.3 ± 0.1 d4 | 14.4 ± 0.0c4 | 3.5 ± 0.0 b3 | 0.0 ± 0.0 e | 541877*** |
10hrs | 59.1 ± 0.0a5 | 87.0 ± 0.3 b5 | 87.4 ± 0.4 b4 | 99.5 ± 0.0 c5 | 4225*** | 99.5 ± 0.0e5 | 99.6 ± 0.0e5 | 28.2 ± 0.7 d5 | 14.4 ± 0.1c4 | 3.5 ± 0.0 b3 | 0.0 ± 0.0 a | 27423*** |
12hrs | 66.5 ± 0.3a6 | 92.0 ± 0.0 b6 | 94.1 ± 1.2 c5 | 100 ± 0.0d6 | 575*** | 100 ± 0.0e6 | 99.7 ± 0.0e5 | 32.5 ± 1.0 d6 | 14.4 ± 0.1c4 | 3.5 ± 0.0b3 | 0.0 ± 0.0 a | 12754*** |
F-value | 35472*** | 5096*** | 1952*** | 97832*** | Â | 97832** | 2184*** | 418*** | 663*** | 2413*** | Â | Â |
Completely decolorize | 72hr | 30hr | 24hr | Â | Â | Â | After 13hrs | After 72hrs | Â | Â | Â | Â |