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Fig. 3 | BMC Biotechnology

Fig. 3

From: Hypoxia and HIF-1 inhibition enhance lentiviral transduction efficiency: a novel strategy for gene delivery optimization

Fig. 3

PX-478 reverses hypoxia-induced inhibition and enhances viral genome integration. (A) CCK-8 assay of PX-478-treated K562 cells. n = 6. (B) mRNA expression levels of HIF1A and GLUT1 with or without PX478 treatment and under hypoxia. n = 3. (C, D, E) Fluorescence images, flow cytometry plots, and quantification of infected cells with or without PX-478 pretreatment. n = 3. (F) qPCR analysis of viral genome integration. n = 3. ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, data are presented as the mean ± standard deviation

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BMC Biotechnology

ISSN: 1472-6750

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