Table 1 Primers used in qPCR analysis –18S forward –18S reverse
Target | Human sequence |
|---|---|
MMP-2 forward | 5’ GGAGACAAGTTCTGGAGATACAATG 3’ |
MMP-2 reverse | 5’ TTTGGTTCTCCAGCTTCAGGTA 3’ |
MMP-9 forward | 5’ GTTCCCGGAGTGAGTTGAAC 3’ |
MMP-9 reverse | 5’ TTTACATGGCACTGCCAAAGC 3’ |
GAPDH forward | 5’ CAAGGAGTAAGACCCCTGGA 3’ |
GAPDH reverse | 5’ AGGGGAGATTCAGTGTGGTG 3’ |
18 S forward | 5’ GTAACCCGTTGAACCCCATT 3’ |
18 S reverse | 5’ CCATCCAATCGGTAGTAGCG 3’ |