Fig. 4

Effect of collagen I on MMP2 and MMP9 mRNA expression in kidney cancer spheroids. Five spheroids were pooled and cultured for 24 h with or without collagen I and MMP2 and MMP9 mRNA levels were measured by RT-qPCR. Levels of MMP9 transcript in 786-O (A) or WT-CLS1 (C) spheroids stimulated or not by collagen I. Levels of MMP2 transcript in 786-O (B) or WT-CLS1 (D) spheroids stimulated or not by collagen I. For each independent experiment, the mean value of the triplicate without collagen I was used for normalization. Data are presented as the mean +/-SEM of 4 to 5 independent experiments (n = 5 for 786-O cells and n = 4 for WT-CLS1 cells). **p < 0.01, Wilcoxon-Mann-Whitney test