Extract optimization and biological activities of Otidea onotica using Artificial Neural Network-Genetic Algorithm and response surface methodology techniques

Table 1 TAS values of Otidea onotica extracts

Experiment number	Extraction temperature (°C)	Extraction time (h)	Ethanol/water ratio (%)	TAS (mmol/L)
1	45	5	0	4.231 ± 0.050^cd
2	45	10	0	6.071 ± 0.042^hi
3	45	15	0	3.890 ± 0.023^a
4	45	5	0	4.245 ± 0.038^d
5	45	10	0	6.033 ± 0.054^h
6	45	15	0	3.910 ± 0.039^a
7	45	5	0	4.196 ± 0.061^bcd
8	45	10	0	6.108 ± 0.017ⁱ
9	45	15	0	3.870 ± 0.039^a
10	55	5	50	6.574 ± 0.012^j
11	55	10	50	7.360 ± 0.024^k
12	55	15	50	5.840 ± 0.027^g
13	55	5	50	6.555 ± 0.029^j
14	55	10	50	7.344 ± 0.016^k
15	55	15	50	5.852 ± 0.009^g
16	55	5	50	6.553 ± 0.029^j
17	55	10	50	7.360 ± 0.029^k
18	55	15	50	5.854 ± 0.037^g
19	65	5	100	4.934 ± 0.019^e
20	65	10	100	5.345 ± 0.022^f
21	65	15	100	4.180 ± 0.019^bc
22	65	5	100	4.943 ± 0.027^e
23	65	10	100	5.346 ± 0.018^f
24	65	15	100	4.170 ± 0.013^b
25	65	5	100	4.958 ± 0.033^e
26	65	10	100	5.335 ± 0.019^f
27	65	15	100	4.162 ± 0.021^b

^aMeans with different superscript letters in the same column are significantly different (p < 0.05) according to Duncan’s multiple range test

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