Fig. 5

Phylogenetic Tree of Aspergillus niger MN. The phylogenetic tree is based on the partial sequence of the small subunit ribosomal RNA gene, complete sequence of the internal transcribed spacer 1 and 5.8 S ribosomal RNA gene, and partial sequence of internal transcribed spacer 2. The ITS sequences were deposited in the GenBank database (http://blast.ncbi.nlm.nih.gov/, accessed on 10 July 2024) to identify closely related species. They were used to build a maximum likelihood phylogenetic tree with the default multiple sequence alignment methods, and the tree inference method was FastTree 2.1.1 builder (Price et al., 2009) in Geneious 9.1.8 software (http://www.geneious.com/). Branch lengths are proportional to nucleotide substitutions/site, and trees were visualized in iTOL v6.3 software (https://itol.embl.de/)