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Table 4 Comparison of fed-batch and chemostat cultivations. µ/D: values in percent relative to the maximum specific growth rate µ [h-1]. For control run A and B a range is given due to a constant feeding profile. qs [g*g-1*h-1]: Values in percent relative to the maximum specific substrate uptake rate. For control run A and B at the beginning of the constant feeding profile is given. Specific productivity (qp) [g*g-1*h-1]: Values are normalized to the achieved specific product formation rate in control run A at the end of the fermentation. The depicted values are the average for the whole process as soon as constant process conditions had been achieved. Space-time yield (STY) [g*L-1*h-1]: Values are the overall STY for the total process duration for fed-batch and the average in the constant phase for chemostats. All values are normalized to the achieved STY in control run A

From: Transferability of bioprocessing modes for recombinant protease production: from fed-batch to continuous cultivation with Bacillus licheniformis

 

Fed-batch cultivations

Continuous cultivations

control run A

control run B

Screening 1

Screening 2

Chemostat 1

Chemostat 2

Chemostat 3

Chemostat 4

Chemostat 5

Chemostat 6

µ/D [%]

16 – 8

15 - 9

48

18

24

39

72

19

49

57

Process time for calculation [h]

50 - 81

50 - 81

25 - 38

25 - 55

33 - 218

32 - 217

199 - 295

56 - 218

49 - 217

141 - 295

qs [%]

12*

12*

48 +/- 1

18 +/- 2

32 +/- 7

50 +/- 4

98 +/- 5

25 +/- 2

60 +/-3

78 +/- 6

qp [%]

95 +/- 21

83 +/- 59

119 +/- 31

141 +/- 22

56 +/- 56

69 +/- 17

0

29 +/- 32

43 +/- 19

2.5 +/- 5

STY [%]

94

84

80

131

30 +/- 22

46 +/- 11

0

21 +/- 16

33 +/- 10

2 +/- 3