Fig. 5

Overexpression of TNFSF4 weakened the protective effects of miR-125b-5p against ox-LDL-mediated HUVEC injury. (A-B) The overexpression of TNFSF4 at mRNA and protein mRNA and protein levels was confirmed in ox-LDL-treated HUVECs after transfection with Ov-TNFSF4. ***p < 0.001, compared with Ov-NC; HUVECs were transfected with miR-125b-5p mimics alone or in combination with Ov-TNFSF4 for 48 h, followed by treated with 100 µg/ml of ox-LDL for 24 h, which were applied for CCK-8 assay (C), ELISA assay (D), oxidative stress assay (E), flow cytometry assay (F) and capillary-like network formation assay (G), respectively. All experiments were carried out three times with three repetitions. ^**p < 0.01, ^***p < 0.001, compared with miR-NC; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, compared with miR-125b-5p mimics + Ov-NC