Fig. 3

Overexpression of miR-125b-5p alleviated ox‑LDL‑induced damage in HUVECs. HUVECs were transfected with miR-125b-5p mimics or miR-NC for 48 h, followed by treated with 100 µg/ml of ox-LDL for 24 h. (A) The expression of miR-125b-5p was determined using quantitative real-time PCR analysis. (B) Cell viability of HUVECs was assessed using CCK-8 assay. (C) The concentrations of inflammation-associated cytokines (TNF-α and IL-1β) were detected by ELISA. (D) The production of SOD and MDA was assessed using their matching kits. (E) Cell apoptosis was determined using flow cytometry assay. (F) The ability of tube formation was analyzed through capillary-like network formation assay in vitro. All experiments were carried out three times with three repetitions. ^**p < 0.01, ^***p < 0.001, compared with control; ^##p < 0.01, ^###p < 0.001, compared with ox-LDL + miR-NC