Fig. 1

Expression levels of miR-125b-5p, TNFSF4 and TLR4/NF-κB signaling factors in an in vitro AS model. HUVECs were exposed to diverse concentrations (25, 50, 100 µg/ml) of ox-LDL for 24 h. (A) Cell viability of HUVECs was determined using CCK-8 assay. (B-C) The expression of miR-125b-5p and TNFSF4 mRNA was measured using quantitative real-time PCR analysis in HUVECs. (D) The protein levels of TNFSF4, TLR4, p-p65, p65, p-IκBα and IκBα were visualized and quantified via western blot analysis in HUVECs. All experiments were carried out three times with three repetitions. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, compared with 0 µg/ml