Table 9 Purification steps of PGase production by Aspergillus fumigatus BT-4
Sr. No | Purification Steps for Pectinase | Total enzyme activity (IU) | Total protein content (mg/mL) | Specific activity (U/mg) | Purification fold | % Yield |
|---|---|---|---|---|---|---|
1 | Crude | 2400 | 76.4 | 31.4 | 1 | 100 |
2 | Ammonium sulphate precipitation | 1518 | 42 | 36.1 | 1.15 | 63.25 |
4 | Dialysis | 975 | 24 | 40.6 | 1.29 | 64.22 |
5 | Ion-exchange chromatography | 529 | 11 | 48.0 | 1.53 | 54.25 |
6 | Gel-Filtration | 233 | 3.2 | 72.8 | 2.31 | 44.04 |