Statistical optimization of pectinases from thermophilic Aspergillus fumigatus BT-4 employing response surface methodology through submerged fermentation using agricultural wastes

Table 7 Analysis of Variance for quadratic model of PGase activity of A. Fumigatus BT-4

Source	Sum of squares	df	Mean square	F-value	p-value
Model	470.22	9	52.25	21.72	0.0003	Significant
A-Temperature	1.45	1	1.45	0.6016	0.4634
B-pH	253.69	1	253.69	105.45	< 0.0001
C-inoculum age	31.69	1	31.69	13.17	0.0084
AB	2.45	1	2.45	1.02	0.3469
AC	18.57	1	18.57	7.72	0.0274
BC	1.47	1	1.47	0.6102	0.4603
A²	67.39	1	67.39	28.01	0.0011
B²	161.64	1	161.64	67.19	< 0.0001
C²	33.29	1	33.29	13.84	0.0075
Residual	16.84	7	2.41
Lack of fit	16.17	5	3.23	9.70	0.0961	Not significant
Pure error	0.6667	2	0.3333
Cor total	487.06	16

Model F-value = 21.72, R-squared = 0.9654, Adjusted R- squared = 0.9210, Predicted R-squared 0.7231, Adequate precision ratio = 14.2196

ISSN: 1472-6750