Table 4 PCR program for amplification of all used genes

PCR

Initial denaturation

94 °C

2 min

35cycles

Denaturation

94 °C

30 s

Annealing

58 °C

30 s

Extension

72 °C

30 s

Final extension

72 °C

3 min

Cooling

4 °C

∞

qPCR

UDG enzyme actiton

37 °C

120 s

Prenaturation

95 °C

1 min

40cycles

Denaturation

95 °C

10 s

Annealing/Extension

60 °C

20 s