Table 2 Key characteristics of the four selected DNA extraction procedures used in this study
Extraction protocol | Kit name abbreviation | Cell lysis | Extraction buffer | Elution buffer | DNA purification | Special advantages | |
|---|---|---|---|---|---|---|---|
Noncommercial method | Modified CTAB method | MC | CTAB | 20 g/L CTAB、1.4 mol/L NaCl、0.02 mol/L EDTA(pH8.0)、0.1 mol/L Tris-HCL(p H8.0) | 50 µL TE (pH 8.0) | Isopropanol | Economical; widely used |
Commercial methods | Plant Genomic DNA Kit | PG | CTAB | 700 µL 65 °C preheated GP1 and 0.1% β-mercaptoethanol | 50–200 ul TE | Spin Columns CB3 | Fast; simple; convenient; widely used |
Magnetic Plant Genomic DNA Kit | MPG | SDS, RNase A | 400 ul buffer GPM and 5 ul RNase A (10 mg/ml) | 50–100 ul TB | Isopropanol/MagAttract Suspension G | Rapid and convenient | |
Combined method | isopropyl alcohol precipitation combined with Processed Food DNA Extraction Kit | IPF | Proteinase K, SDS | 500 ul buffer GMO1 and 20 ul Proteinase K (20 mg/ml) | 20–50 ul TE | Isopropanol | Safe; convenient; excellent scalability and flexibility |