Fig. 5
From: Metabolic studies of Ogataea polymorpha using nine different corn steep liquors
Influence of ammonium limitation on the cell size distribution of Ogataea polymorpha cultivations in RAMOS flasks. (A) Oxygen transfer rate (OTR) of O. polymorpha RB11 pC10-FMD (PFMD-GFP). Data points shown are mean values, ± half the amplitude between duplicate cultivations is shown as shadows. OTR was measured until the end of experiment, 8.5 h. (B-D) Cell size distribution obtained after sampling at hour 5, 7 and at the end of the cultivation (8.5 h). Experimental parameters: 250 mL RAMOS flask, CSL 1 concentration \(\:{c}_{CSL,1}\) = 2.5 g dry substance/L, glucose concentration \(\:{c}_{glucose}\) = 5 g/L, ammonium sulfate concentration \(\:{c}_{(N{H}_{4}{)}_{2}S{O}_{4}}\) = 0 and 0.6 g/L, MES buffer concentration \(\:{c}_{MES}\) = 0.1 M, initial pH = 6.0, culture volume VL = 10 mL, shaking frequency n = 350 rpm, shaking diameter d0 = 50 mm, temperature T = 37 °C and initial OD600 = 0.1