Fig. 2

Influence of different corn steep liquor (CSL) 1 concentrations on Ogataea polymorpha cultivations in a µTOM device. (A) Oxygen transfer rate of O. polymorpha RB11 pC10-FMD (PFMD-GFP). All data points are mean values from 11–12 individually measured wells. Shadows indicate the respective standard deviation. For some CSL variants, the respective shadows are barely visible, because of low standard deviations < 0.5. (B) The three parameters optical density at 600 nm wavelength (OD₆₀₀), total oxygen (TO) and final pH values, were measured at the end of experiment, after 19 h. OD₆₀₀ and final pH values were obtained in triplicates, whereas the mean value for TO was calculated from OTRs in duplicate cultivations. Error bars for OD₆₀₀ and final pH values in (B) indicate the respective standard deviation. Error bars for final pH values are barely visible, due to low standard deviations < 0.5. Significant differences to the reference (5 g/L CSL 1) are marked with asterisks, corresponding to the significance level (* for 0.01 < p < 0.05, ** for 0.001 < p < 0.01 and *** for p < 0.001). Experimental parameters: round deep-well 96-well microtiter plate (MTP), CSL 1 concentration c_CSL,1 = 1.25–10 g dry substance/L, glucose concentration c_glucose = 5 g/L, MES buffer concentration c_MES = 0.1 M, initial pH = 6.0, culture volume V_L = 200 µL/well, shaking frequency n = 350 rpm, shaking diameter d₀ = 50 mm, humidity = 80%, temperature T = 37 °C and initial OD₆₀₀ = 0.1