Table 1 Strains and plasmids used in this study
Strains or plasmids | Descriptions | References |
|---|---|---|
Strains | ||
 E. coli JM109 | Clone strain | Takara |
 A. niger MA70.15 | ∆kusA, ∆pyrG, clone strain | Our laboratory |
 AnGggL | A.niger MA70.15/pCGgg-lcc9, PgpdA, SPGlaA | This study |
 AnGgeL | A.niger MA70.15/pCGge-lcc9, PgpdA, SPCBHI | This study |
 AnGgoL | A.niger MA70.15/pCGgo-lcc9, PgpdA, SPORZ | This study |
 AnGguL | A.niger MA70.15/pCGgu-lcc9, PgpdA, SPBGL | This study |
 AnGgxL | A.niger MA70.15/pCGgx-lcc9, PgpdA, SPBxyA | This study |
 AnGgnL | A.niger MA70.15/pCGgn-lcc9, PgpdA, SPExyA | This study |
 AnGgdL | A.niger MA70.15/pCGgd-lcc9, PgpdA, SPEGII | This study |
 AnGgyL | A.niger MA70.15/pCGgy-lcc9, PgpdA, SPEXY | This study |
 AnGgcL | A.niger MA70.15/pCGgc-lcc9, PgpdA, SPCAT | This study |
 AnGccL | A.niger MA70.15/pCGcc-lcc9, PcitA, SPCAT | This study |
 AnGscL | A.niger MA70.15/pCGsc-lcc9, PsucA, SPCAT | This study |
 AnGmcL | A.niger MA70.15/pCGmc-lcc9, PmbfA, SPCAT | This study |
 AnGacL | A.niger MA70.15/pCGac-lcc9, Pcat, SPCAT | This study |
 AnGcgL | A.niger MA70.15/pCGcg-lcc9, PcitA, SPGlaA | This study |
 AnGsgL | A.niger MA70.15/pCGsg-lcc9, PsucA, SPGlaA | This study |
 AnGmgL | A.niger MA70.15/pCGmg-lcc9, PmbfA, SPGlaA | This study |
 AnGagL | A.niger MA70.15/pCGag-lcc9, Pcat, SPGlaA | This study |
 AnCcgL | A.niger MA70.15/pCCcg-lcc9, PcitA, SPGlaA | This study |
 AnRcgL1 | A.niger MA70.15/pCRcg-lcc9, PcitA, SPGlaA | This study |
Plasmids | ||
 pC3 | Recombinant expression vector backbone, pyrG | Our laboratory |
 pUC57-lcc9 | ampR (E. coli), lcc9 | Sangon Biotech |
 pUC57-SPCBHI | ampR (E. coli), SPCBHI | Our laboratory |
 pUC57-SPORZ | ampR (E. coli), SPORZ | Our laboratory |
 pUC57-SPBxyA | ampR (E. coli), SPBxyA | Our laboratory |
 pUC57-SPBGL | ampR (E. coli), SPBGL | Our laboratory |
 pUC57-SPExyA | ampR (E. coli), SPExyA | Our laboratory |
 pUC57-SPEGII | ampR (E. coli), SPEGII | Our laboratory |
 pUC57-SPEXY | ampR (E. coli), SPEXY | Our laboratory |
 pUC57-SPCAT | ampR (E. coli), SPCAT | Our laboratory |
 pCGgg-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPGlaA-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGge-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPCBHI-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGgo-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPORZ-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGgu-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPBGL-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGgx-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPBxyA-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGgn-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPExyA-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGgd-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPEGII-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGgy-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPEXY-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGgc-lcc9 | pC3 derivative, pC3-5′glaA-PgpdA-SPCAT-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGcc-lcc9 | pC3 derivative, pC3-5′glaA-PcitA-SPCAT-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGsc-lcc9 | pC3 derivative, pC3-5′glaA-PsucA-SPCAT-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGmc-lcc9 | pC3 derivative, pC3-5′glaA-PmbfA-SPCAT-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGac-lcc9 | pC3 derivative, pC3-5′glaA-Pcat-SPCAT-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGcg-lcc9 | pC3 derivative, pC3-5′glaA-PcitA-SPGlaA-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGsg-lcc9 | pC3 derivative, pC3-5′glaA-PsucA-SPGlaA-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGmg-lcc9 | pC3 derivative, pC3-5′glaA-PmbfA-SPGlaA-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCGag-lcc9 | pC3 derivative, pC3-5′glaA-Pcat-SPGlaA-lcc9-TtrpC-pyrG-3′glaA | This study |
 pCCcg-lcc9 | pC3 derivative, pC3-5′cat-PcitA-SPGlaA-lcc9-TtrpC-pyrG-3′cat | This study |
 pCRcg-lcc9 | pC3 derivative, pC3-PcitA-SPGlaA-lcc9-TtrpC-pyrG | This study |