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Table 2 Validation test of virus stabilities following the virus spike of sample material

From: Chemical and Heat Treatment for Viral Inactivation in Porcine-Derived Gelatin

Virus

Spiked

(log10TCID50/mL)

Recovered

(log10TCID50/mL)

Recovery error

Canine parvovirus (CPV)

7.5 ± 0.1

5.6 ± 0.2

1.9

Bovine adenovirus (BAV)

7.9 ± 0.0

6.2 ± 0.1

1.7

Bovine parainfluenza (BPIV)

9.0 ± 0.1

7.0 ± 0.2

2.0

Pseudorabies virus (PRV)

8.6 ± 0.0

7.8 ± 0.0

0.8

Bovine reovirus (BReoV)

8.5 ± 0.14

8.7 ± 0.07

0.2

Porcine parvovirus (PPV)

7.9 ± 0.0

7.9 ± 0.07

0.0

  1. The spike virus was added at a 1:10 ratio. For the validation samples, the virus quantity was measured after processing the emulsion, which included a final dilution factor of 10. The validation samples, after correction, accounted for a 100-fold increase by adding ‘2’ to the value to adjust for the dilution
  2. The Recovery Error refers to the absolute value of the difference between the virus quantity measured in the supernatant and the amount of spiked virus added to the samples, as calculated based on the spike virus (working sheet)