Fig. 1

Workflow of the MAC cloning procedure. A Cells are resuspended. This step is critical, particularly in cell cultures that tend to aggregate. B 100 to 200 cells are deposited in each well containing 550 low-adhesion cavities. C Cells are incubated at 37⁰C for 10 days until the spheroids form. D Clone recovery. Using a standard laboratory pipet, spheroids can be transferred to a standard cell culture plate with the adhesive surface for outgrowth and testing