Fig. 2

(a) Growth inhibition of Escherichia coli using different concentrations of multi-enzyme from 1 to 6 U/mL after incubation at 37 °C for 12 h. A negative control, consisting of an Escherichia coli suspension in LB broth without the enzyme, was also included in the assay. Means ± standard deviations (n = 3). Values with different letters differ significantly (p < 0.05). (b) Zone of inhibition diameter against Escherichia coli as a result of multi-enzyme activity (diluted enzyme mixture (E1) and undiluted enzyme mixture (E2)) incubated at 37 °C for 24 h. Autoclaved distilled water served as the negative control and kanamycin (50 mg/mL) was used as the positive control