Table 4 Reaction flow and reaction parameters
Reaction step | Contents | parameters |
|---|---|---|
Step 1 | Add calibrators, samples, quality controls | 30 μL |
Step 2 | Adding a reducing agent | 15 μL |
Step 3 | Mix well and react in a warm bath | 37℃, 5 min |
Step 4 | Add enzyme | 15 μL |
Step 5 | Magnetized particulate reagent | 30 μL |
Step 6 | Addition of anti-reagents | 30 μL |
Step 7 | Mix well and react in a warm bath | 37℃, 30 min |
Step 8 | Add cleaning solution for chemiluminescence system and mix well. | 300 μL |
Step 9 | Repeat step 8 for magnetic separation of the reactants | 3 times |
Step 10 | Addition of chemiluminescent substrate solution for fully automated immunoassay for luminescence detection. | 200 μL |