Table 2 Optimization of the antibody concentration
Anti-SAH-AP (µg/mL) | 0.1 | 2.0 | 5.0 | ||||||
|---|---|---|---|---|---|---|---|---|---|
DTT (µg/mL) | 0.1 | 2.0 | 5.0 | 0.1 | 2.0 | 5.0 | 0.1 | 2.0 | 5.0 |
Standard 1 (0 µmol/L) | 349377 | 360657 | 355173 | 360610 | 727219 | 884461 | 356137 | 727837 | 958345 |
Standard 2 (2.5 µmol/L) | 279598 | 290191 | 279092 | 298932 | 554226 | 625298 | 308952 | 543313 | 830502 |
Standard 3 (50 µmol/L) | 53040 | 64025 | 66878 | 69015 | 95542 | 199266 | 71169 | 103976 | 329685 |
SAHase (µg/mL) | 0.1 | 2.0 | 3.0 | ||||||