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Table 2 Effect of incubation time on the different types of cellular extract efficiency, testololactone coefficient yield (Ypt/s), volumetric testololactone productivity (Qpt), and average progesterone transformation rate (vpg) by P. chrysogenum Ras3009.

From: Enhancing the biotransformation of progesterone to the anticancer compound testololactone by Penicillium chrysogenum Ras3009: kinetic modelling and efficiency maximization

Time (h)

WHCE

(%)

ICE

(%)

ECE

(%)

Ypt/s

(mg/mg)

Qpt

(%)

vpg

(mgL− 1h− 1)

12

40.90a ± 1.17

29.21a ± 0.21

8.02a ± 0.07

0.355a

32.62a ± 0.19

37.50a ± 0.18

18

61.81b ± 1.20

50.22b ± 0.22

12.15b ± 0.09

0.537b

32.86a ± 0.18

37.77a ± 0.17

24

74.54cb ± 1.18

65.11c ± 0.18

14.18b ± 0.08

0.648c

29.72ab ± 0.19

34.16ab ± 0.19

30

87.72cd ± 0.10

78.12cd ± 0.19

18.12c ± 0.09

0.763d

27.98abc ± 0.21

32.16ab ± 0.18

36

92.00d ± 0.10

82.00d ± 0.22

19.34cd ± 0.08

0.800d

24.45bcd ± 0.16

28.11b ± 0.18

42

100.00d ± 0.0

89.00d ± 0.23

21.15cd ± 0.09

0.870d

22.78bd ± 0.18

26.19bc ± 0.17

48

100.00d ± 0.0

91.12d ± 0.22

22.45d ± 0.11

0.870d

19.93de ± 0.19

22.91cd ± 0.19

54

100.00d ± 0.0

93.00d ± 0.21

23.11d ± 0.11

0.870d

17.72e ± 0.17

20.37d ± 0.11

  1. HCE; Whole cell extract efficiency (%), ICE; Intracellular extract efficiency (%), ECE; Extracellular extract efficiency (%), Ypt/s; Testololactone coefficient yield (mg/mg), Qpt; Volumetric testololactone productivity (mgL-1h-1), Qs; Average progesterone transformation, (mgL-1h-1). Values are means of three replicates; values followed by same letters on the same column are not significantly different (P < 0.005) in Tukey’s test