Fig. 4
From: Establishment of a novel cell line for producing replication-competent adenovirus-free adenoviruses
E1 mRNA expression in transfected cells with retroviral vector. Total RNA from HT1080 (HT), HEK293A (293A), or pWZL-E1-transfected HT1080 (HT/E1) cells was reverse-transcribed into cDNA. The DNA of the genomic E1-encoded vector, pWZL-gE1 (gE1), was included as a control. Genes were amplified via PCR. Uncropped gel is presented supplementary Fig. 2