Fig. 5

Determination of protein levels of the pro-inflammatory cytokines TNF-α and IL-6 in the cell culture medium of the LPS-stimulated RAW 264.7 macrophages using ELISA. The selected fungal extracts (Sh F and St Cell in medium B and Sh Cell in medium C) showed a significant reduction of the LPS-stimulated upregulation of the pro-inflammatory cytokines TNF-α (A) and IL-6 (B). Results are presented as the mean ± SE (n = 3). Statistical significance was calculated by one-way ANOVA followed by Student–Newman–Keuls post-hoc test. $ P < 0.05 vs. control cells. * P < 0.05 vs. LPS-stimulated cells