Table 1 Strains and plasmids used in this study
Strains or plasmids | Genotype or characterization | Source or reference |
|---|---|---|
Escherichia coli | ||
DH5α | F−, φ 80dlacZΔM15, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rK−,mK+), phoA, supE44, λ−, thi − 1, gyrA96, relA1 | TaKaRa Bio Inc., Dalian, China |
Glaesserella parasuis | ||
SH0165 | Wild-type, serotype 5 | [22] |
JS0135 | Wild-type, serotype 4 | This laboratory |
JS0135ΔnanH::KanR | ΔnanH::KanR, generated via natural transformation of pK18-nHUD | This laboratory |
CF7066ΔnanH-N | ΔnanH, constructed by NgAgo deletion system | This study |
JS0135ΔnanH | ΔnanH, constructed by temperature-sensitive system | This study |
CF7066 | Wild-type, serotype 5 | This laboratory |
CF7066ΔnanH | ΔnanH, generated via temperature-sensitive system | This study |
CF7066Δapd | Δapd, generated via temperature-sensitive system | This study |
Plasmids | ||
pK18 mobsacB | pUC ori, KanR, suicide vector of G. parasuis | [23] |
pK18-nHUD | pUC ori, KanR, ΔnanH, from pK18 mobsacB, containing upstream and downstream regions of nanH to be deleted | This study |
pSHK3-Gm | GmR, pUC ori, pYC93 ori, shuttle vector of G. parasuis | [18] |
pSHK3TS | KanR, pUC ori, pA13 oriTS, temperature-sensitive vector of G. parasuis | [17, 24] |
pSHG5 | GmR, pUC ori, pA13 oriTS | This study |
pSHG5-ΔnanH | GmR, pUC ori, pA13 oriTS, ΔnanH | This study |
pSHG5-Δapd | GmR, pUC ori, pA13 oriTS, Δapd | This study |