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Fig. 2 | BMC Biotechnology

Fig. 2

From: Purification and characterization of soluble recombinant Crimean-Congo hemorrhagic fever virus glycoprotein Gc expressed in mammalian 293F cells

Fig. 2

A. Schematic of the plasmid: Gc-ecto(Ins) represents construct 3 in Fig. 1A. B-C. Analysis of CCHFV Gc under non-reducing conditions: Supernatant from construct 3 transfected 293 F cells was passed through a nickel agarose column and the eluted protein was analyzed by SDS-PAGE (B) and western blotting (C). D-E: Analysis of CCHFV Gc under reducing conditions. Supernatant from construct 3 transfected 293 F cells was passed through a nickel agarose column, the eluted protein was treated with the reducing agent DTT and analyzed by SDS-PAGE (D) and western blotting (E). F: Analysis of CCHFV Gc by Native PAGE. Supernatant from construct 3 transfected 293 F cells was passed through a nickel agarose column, the eluted protein was treated with 1% triton X-100 and resolved on a 4–12% native PAGE gel. PageRuler™ Pestained Protein Ladder (#26616;ThermoFisher Scientific, UK) was used to estimate the size of the protein on the SDS-PAGE gels and Western blot membranes

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BMC Biotechnology

ISSN: 1472-6750

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