Fig. 7

Regulation of cell viability, migration, invasion and angiogenic capacity by knockdown and overexpression of PAR-1. A EDU examined cell viability and found that knockdown of PAR-1 reduced cell viability, with the opposite result for overexpression, scale bar = 20 μm. B Transwell assayed the invasive capacity of the cells, and the trend after knockdown and overexpression of PAR-1 was consistent with EDU. C A cell wound healing assay tested the migratory capacity of cells and found that PAR-1 regulated the migratory capacity. D Angiogenesis experiments demonstrated changes in the number of blood vessels following alterations in PAR-1 expression, x100. One-way ANOVA was used for comparison between multiple groups. n.s, not significant, ****P < 0.0001, ***P < 0.001 and **P < 0.01