Fig. 5

Effect of knockdown and overexpression of PAR-1 on M2 macrophage. A Western blotting examined the efficiency of transfection of sh-PAR-1 and pcDNA3.1-PAR-1. The image showed a cropped blot, the original blot was included in the Supplementary file. B Effect on cell polarisation after transfection with sh-PAR-1 and pcDNA3.1-PAR-1. The supporting images of the gating strategy are shown in Supplementary Fig. S1D. C Statistical results of cell polarisation markers from flow cytometry. One-way ANOVA was used for comparison between multiple groups. n.s, not significant, ****P < 0.0001, ***P < 0.001 and **P < 0.01