Fig. 3
From: Recombinant human enamelin produced in Escherichia coli promotes mineralization in vitro
Column-based purification of enamelin. (A) Chromatograms showing purification of enamelin using different imidazole concentrations (0–30 mM) during loading and washing steps. Main elution peaks are shown (grey box). (B) SDS-PAGE analysis of the gradient peak fraction from 0 mM experiment. (C) Elution fractions from the column purifications analyzed with SDS-PAGE. The arrow indicates the enamelin band. Imidazole concentrations during binding and washing are indicated above each corresponding well