Fig. 2
From: Recombinant human enamelin produced in Escherichia coli promotes mineralization in vitro
SDS-PAGE analysis of batch-mode enamelin purification. (A) Purification of enamelin was carried out using four different metal ions (Ni2+, Cu2+, Co2+, Zn2+) with an imidazole concentration of 30 mM during binding and washing. The image shows elution fractions from using the metal ions indicated above each corresponding well. (B) Elution fractions from Ni2+-based purifications of enamelin using different imidazole concentrations (0–50 mM) during binding and washing. The concentration is indicated above each corresponding well