Fig. 3

Double and triple NLS variants of Bxb1 integrase. Construct on top shows the configration of the double NLS variants with NLSs at N- and C-terminus. Constructs in the middle and at the bottom illusrate the configuration of Bxb1-NPLCx2 and Bxb1-NPLCx3 variants, respectively. NLS sequences of Bxb1-NPLCx2 and Bxb1-NPLCx3 are separated by a flexible GGGGSGGGGSGS linker (white arrow)