Fig. 1

Mycobacteriophage Bxb1 serine integrase with N-terminal and C-terminal nuclear localization signal fusions (NLS). Upper construct illustrates Mycobacteriophage Bxb1 serine integrase with N-terminal NLS fusion. N-terminal NLS (yellow) and Mycobacteriophage Bxb1 serine integrase gene (blue) was separated by a GS linker (orange) and human influenza hemagglutinin (HA) epitope tag (red). Lower construct illustrates Mycobacteriophage Bxb1 serine integrase with C-terminal NLS. Amino acid sequences of the NLSs are shown below the constructs