Fig. 4

In vitro validation data (A) hBMP2 ELISA data from HEK293T cells. All computationally selected sequences showed non-significant increases in secretion. N = 7 ± SD, **** = p < 0.0001 (B) hBMP2 ELISA data from C2C12 cells. All computationally selected sequences showed decreased secretion versus the positive control, with several showing significant decreases. Particularly notable were the sequences that showed major decreases in the C2C12 results compared to the HEK293T experiment. These were SP1, SP2, SP hIL2 and SP hBMP2 TIS. N = 3 ± SD. * = p < 0.05, ** = p < 0.01, *** = p < 0.001 C) ALP assay data from C2C12 cells. All groups except the positive control showed background levels of ALP activity, indicating the novel SPs were not sufficient to induce osteogenesis in this context. N = 3 ± SD, *** = p < 0.001