Fig. 1

Surgical procedure for the induction of volumetric muscle loss in a mouse model after biceps femoris muscle injury and transplantation of myoblast-laden Col/Keratin hydrogel. After shaving and disinfection, a surgical incision was made to get access to the biceps femoris muscle mass. For volumetric muscle loss, segments of skeletal muscle surgical were removed surgically. The process followed by the injection of Col, and Col/Keratin hydrogels with or without the myoblasts. After being solidified, the incision line was carefully sutured and the animals were kept for 15 days