Fig. 6

HPLC detection of VB12 production and growth status detection in liquid shake flask cultures for episomal expression and integrated expression strains. A The VB12 yields of the episomal overexpression strain. B The VB12 yields of the integrated expression strain. C Growth curves of the wild-type and recombinant strains. P value < 0.0001 is labelled as ****, P value < 0.001 is labelled as ***, P value < 0.01 is labelled as **, and P value < 0.05 is labelled as *