Skip to main content
Fig. 2 | BMC Biotechnology

Fig. 2

From: Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system

Fig. 2

Electroporation of PFFs using Nucleocuvette™ strips. The transfection efficiencies and cell survival rates after delivery of 1 µg Cas9-EGFP (A) and equimolar amount of pMAX-GFP (B) plasmid via the built-in programs of Lonza basic protocol. (C) Transfection efficiencies and cell survival rates after delivery of Cas9-EGFP plasmid via different dual-electroporation programs. (D) Effect of plasmid dosage on transfection efficiency and cell survival rate. (E) Transfection efficiencies after delivery of 1 µg Cas9-EGFP to different amounts of cells. *: P < 0.05, **: P < 0.01, ns: P ≥ 0.05

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us