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Fig. 4 | BMC Biotechnology

Fig. 4

From: Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein

Fig. 4

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) quality analyses of purified rRBD produced in flasks. (A) SDS-PAGE analysis of purified rRBD samples transiently expressed or stably expressed. Transiently produced rRBD is marked by the promoter driving rRBD expression (CAG or CMV) and whether the oriP/wild-type EBNA1 are present (CAG-E or CMV-E). Stably produced rRBD is marked by clone. (B/C) SDS-PAGE analysis of purified rRBD samples (marked by transient culture or stable clone) without and with PNGaseF digestion (+ P). Samples were run across two gels with transiently produced samples on the left, a molecular weight ladder and PNGase F control in the middle, and stably produced samples on the right

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BMC Biotechnology

ISSN: 1472-6750

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