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Fig. 3 | BMC Biotechnology

Fig. 3

From: Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein

Fig. 3

Titers for flask-scale batches measured by orthogonal analytical methods. Absolute titers (A- transient, B- stable) measured on harvest day supernatant samples by ELISA and BLI for the flask-scale batches. (C) A comparison of absolute titers measured by ELISA for all flask batches per transient culture (-E denotes plasmids with wild-type EBNA1) or stable clone is also shown. Error bars show SEM for transient biological duplicates and fed-batch biological triplicates with error propagated for technical replicates (n = 2 for BLI, n = 3 for ELISA)

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BMC Biotechnology

ISSN: 1472-6750

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