Fig. 5

Effect of IgG mut-B2 on angiogenesis. (A) Cell migration assay used to assess angiogenesis in vitro. Lower right panel: numbers of HUVEC per field. (Original magnifcation, ×200) (B) Tube formation assay used to assess angiogenesis in vitro. Lower right panel: numbers of intersection nodes. (Original magnifcation, ×100) (C) Chorioallantoic membrane (CAM) assay used to assess angiogenesis in vivo. Lower right panel: ratio of vascular area to CAM area