Fig. 3

A Diagrams of mono-cistronic constructs expressing SGLuc-IFNα, SGLuc-IFNβ, IFNα, and IFNβ. In SGLuc-IFN constructs, the native secretion domains of porcine IFNα and IFNβ, pale red and pale green respectively, are replaced with the amino acid sequence for SGLuc. B Western blots of media from transfected cell cultures expressing native porcine IFN and chimeric SGLuc-IFNs using anti-IFNα, anti-IFNβ, and anti-GLuc antibodies demonstrate expression and secretion into cell culture media. Blots are cropped to focus on results and representative of N = 3 replicates; uncropped full-length blots are included in Additional file 1: Fig. S2