Fig. 2
From: Highly enhanced ELISA sensitivity using acetylated chitosan surfaces
Analysis of the anti c-myc-ChBD antibody expressed in mammalian cells. Panel a shows 10% acrylamide gel electrophoresis and Coomassie blue staining. Lanes: 1. anti c-myc- ChBD with β Mercaptoethanol (2 μg); 2. Molecular weight pattern. Panel b shows the ELISA detection of anti-c-myc-ChBD antibody activity compared to the antibody purified from hybridoma 9E10. In panel c cytometry of chitin-coated magnetic particles using the specific anti-c-myc-ChBD antibody is depicted. The negative cytometry control for which we used the anti-c-myc antibody is represented in black; particles tagged with the recombinant anti-c-myc-ChBD antibody are represented in red