Fig. 2

NM IBs isolation and solubilization. a SDS-PAGE analysis of the purified NM IBs after extensive washing. b SDS-PAGE analysis of the solubilized NM from IBs by traditional urea-denatured method with 0–7 mol/L urea. c SDS-PAGE analysis of the solubilized NM protein from IBs by one-step heating method with 0–7 mol/L urea. d The protein solubilization efficiency by two different methods with 0–7 mol/L urea