Fig. 1

The schematic diagram of CRISPR-Cas9 binary vector (BGK041). This system includes 19 bp long Synthetic guide RNA (sgRNA), NOS terminator: NOS terminator is used for the termination signal of the gene expression, small U6 expression cassettes derived from soybean, CaMV 35S: promoter used to express the glufosinate resistant gene, BAR gene: A reporter herbicide resistance gene, LB: left border and RB: right border