Figure 1

Flow chart of the single-primer site-directed mutagenesis method. The parental plasmid is shown in grey color and the two PCR synthesized strands are shown in blue and purple. The letter × marks the position of the mutation.
Flow chart of the single-primer site-directed mutagenesis method. The parental plasmid is shown in grey color and the two PCR synthesized strands are shown in blue and purple. The letter × marks the position of the mutation.