Figure 4
From: Recombinant nucleases CEL I from celery and SP I from spinach for mutation detection
Detection of specific incisions 3' of the mismatched G nucleotide by mass spectrometry. The full length substrate (bottom strand) and the incision product peaks are shown. Design of the heteroduplex substrate used and the expected mass of the product are presented at the bottom. "+1 Da" refers to a single-protonated oligonucleotide ion.