Figure 1

Codon adaptation of the used synthetic DNase I gene. As the codon bias of T. thermophila is different from that of human genes 67% of 283 coding triplets of the original human codons (WT) were changed into codons that are known to be mostly present in highly expressed T. thermophila genes (CO) [13,16]. The adapted codons are marked by gray boxes. The corresponding amino acid sequence is given in single letter code; N-glycosylation sites are highlighted in bold letters, cysteines forming disulfide bridges are underlined.