Figure 1

2-photon excitation and emission properties of GFP and CFP expressed in cells. A) Stable cell lines expressing either CFP or GFP were imaged with the indicated excitation wavelength (in all cases the laser power was 0.95–1.0W). CFP fluorescence was captured using a 480/30 filter and non-descanned detection, GFP fluorescence was captured using a 515/30 filter and non-descanned detection. The amount of fluorescence captured using different excitation wavelengths is shown (relative to the maximum fluorescence). B) Stable cell lines expressing either CFP or GFP were imaged using an 880 nm excitation beam (in all cases the laser power was 0.95–1.0W). Fluorescence was captured using the indicated filters and non-descanned detection. The amount of GFP or CFP fluorescence captured using different filters is shown (relative to the maximum fluorescence).