Table 3 C-Terminal fusion expression comparison
| Â | C-TERMINAL FUSION | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Protein (domain) | V5-H6 | H10 | GFP-H10 | GST-H10 | Trx-H10 | MBP-H10 | Dhfr-H10 | DHFR-H10 | ||||||||
| Â | T | S | T | S | T | S | T | S | T | S | T | S | T | S | T | S |
CASP2 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
CCND2 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
CD44 | 1.37 | 0.72 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
CDK2 | 23.47 | 12.30 | 1.88 | 1.30 | 0.70 | 0.19 | 0.53 | 0.08 | 0.86 | 0.16 | 7.52 | 3.48 | 5.82 | 0.51 | 0.00 | 0.00 |
CDK4 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.13 | 0.00 | 0.00 | 0.00 | 2.07 | 0.44 | 0.00 | 0.00 |
CDKN1B | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 1.59 | 0.61 | 0.00 | 0.00 | 5.76 | 0.00 |
CDKN2A | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 2.59 | 0.56 | 0.00 | 0.00 | 0.48 | 0.22 | 0.00 | 0.00 |
Efna1 | 10.64 | 8.29 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.24 | 0.03 | 2.30 | 1.23 | 0.00 | 0.00 | 5.24 | 0.56 |
Efna1 (EC) | 1.71 | 0.46 | 2.76 | 0.58 | 0.25 | 0.00 | 19.99 | 0.24 | 3.71 | 0.00 | 31.94 | 15.60 | 14.01 | 0.00 | 1.10 | 1.02 |
Efnb2 (EC1) | 7.91 | 0.37 | 0.43 | 0.04 | 0.32 | 0.04 | 0.56 | 0.00 | 2.53 | 0.00 | 6.30 | 0.80 | 0.00 | 0.24 | 5.84 | 0.00 |
Efnb2 (EC2) | 3.57 | 0.38 | 0.52 | 0.11 | 0.54 | 0.06 | 0.00 | 0.00 | 2.08 | 0.00 | 3.53 | 1.72 | 2.59 | 0.00 | 0.00 | 0.00 |
EGFR (TK) | 1.14 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.28 | 0.00 | 12.76 | 1.11 | 3.03 | 0.25 | 2.07 | 0.00 | 0.00 | 0.00 |
Epha2 (LB) | 3.97 | 0.27 | 0.00 | 0.00 | 0.15 | 0.00 | 2.37 | 0.00 | 4.33 | 0.42 | 19.33 | 8.60 | 7.42 | 0.00 | 0.00 | 0.00 |
Ephb2 (LB) | 15.17 | 7.46 | 0.00 | 0.00 | 0.00 | 0.00 | 0.64 | 0.00 | 2.34 | 0.94 | 11.93 | 7.93 | 2.48 | 0.37 | 12.65 | 0.00 |
Ephb2 (SAM) | 0.27 | 0.03 | 0.00 | 0.00 | 0.29 | 0.08 | 43.05 | 0.00 | 0.98 | 0.10 | 156.52 | 20.33 | 33.57 | 7.37 | 2.20 | 0.00 |
Ephb2 (TK) | 43.00 | 1.89 | 8.85 | 0.12 | 0.00 | 0.00 | 205.64 | 2.13 | 24.04 | 0.00 | 49.99 | 3.01 | 27.02 | 1.06 | 0.00 | 0.00 |
Fli1 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 3.11 | 0.00 | 1.10 | 0.00 | 0.00 | 0.00 | 7.14 | 0.00 |
FOS | 4.72 | 0.27 | 0.22 | 0.00 | 0.92 | 0.49 | 0.00 | 0.00 | 0.11 | 0.00 | 1.16 | 0.62 | 0.00 | 0.00 | 4.35 | 0.00 |
GATA2 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
GFP | 8.14 | 1.38 | 1.11 | 0.67 | 12.94 | 9.86 | 2.03 | 2.01 | 21.50 | 4.78 | 118.38 | 40.52 | 4.66 | 2.93 | 9.56 | 2.74 |
GRB2 | 15.66 | 5.41 | 0.71 | 0.17 | 3.55 | 3.02 | 2.43 | 1.70 | 5.78 | 3.48 | 110.84 | 14.48 | 4.83 | 3.04 | 1.30 | 0.00 |
HRAS | 19.21 | 6.44 | 0.29 | 0.25 | 11.49 | 6.46 | 0.56 | 0.39 | 1.06 | 0.37 | 12.32 | 10.07 | 3.21 | 3.01 | 0.75 | 0.57 |
JUN | 6.75 | 0.00 | 0.31 | 0.00 | 0.00 | 0.00 | 0.48 | 0.00 | 1.41 | 0.00 | 8.81 | 0.35 | 0.00 | 0.00 | 7.79 | 0.00 |
MAD | 9.76 | 2.62 | 3.94 | 5.05 | 0.49 | 0.13 | 1.37 | 0.00 | 5.91 | 3.34 | 19.02 | 6.94 | 1.86 | 0.13 | 0.88 | 0.00 |
MAX | 0.00 | 0.00 | 0.00 | 0.00 | 0.71 | 0.53 | 101.26 | 80.86 | 1.59 | 0.62 | 9.70 | 5.17 | 5.82 | 3.48 | 0.94 | 0.00 |
Mdm2 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
Mdm2 (p53-bd) | 1.10 | 0.25 | 0.60 | 0.14 | 3.94 | 0.33 | 2.50 | 0.47 | 69.23 | 3.03 | 19.57 | 3.82 | 5.46 | 0.83 | 0.00 | 0.00 |
MMP1 | 12.51 | 0.85 | 0.00 | 0.00 | 0.00 | 0.00 | 3.27 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 4.29 | 0.00 | 0.52 | 0.00 |
RAF1 (Ras-bd) | 0.48 | 0.06 | 0.00 | 0.00 | 0.45 | 0.16 | 0.96 | 0.19 | 2.66 | 0.71 | 5.83 | 1.63 | 2.08 | 1.07 | 0.00 | 0.00 |
Trp53 | 0.52 | 0.07 | 0.00 | 0.00 | 0.00 | 0.00 | 0.23 | 0.00 | 3.59 | 0.91 | 5.66 | 2.44 | 1.40 | 0.79 | 2.76 | 0.00 |
AVERAGE | 6.37 | 1.65 | 0.72 | 0.28 | 1.23 | 0.71 | 12.94 | 2.94 | 5.75 | 0.69 | 20.21 | 4.99 | 4.37 | 0.85 | 2.29 | 0.16 |