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Figure 2 | BMC Biotechnology

Figure 2

From: Generation of single-copy transgenic mouse embryos directly from ES cells by tetraploid embryo complementation

Figure 2

Embryos derived from Hprt-deficient ES cells by tetraploid aggregation. A-C) Photographs of embryos at E13.0 derived from F3 cells. Pigmentation can be seen in the eyes (arrow) reflecting their ES cell origin. D) Southern blot analyses of BamH1-digested genomic DNA isolated from five (#1 – #5) F3 ES cell-derived embryos that was hybridized to a radiolabeled intron 3 probe. Only the mutant allele is detected in F3 ES cell derived embryos. The wild type allele can be seen in female F3 offspring as discussed in the text.

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BMC Biotechnology

ISSN: 1472-6750

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